Plant

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Best Composite Part

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Sequence Feature Legend

T7 / Lac Promoters
Ribosome Binding Site (RBS)
Signal Peptides (pelB)
Enzymes / Domains (ICCG, TfCa)
Linkers / Spacers
Interaction Modules (Dockerin / Cohesin)
Cleavage Sites (TEV) & Purification (Histag)
Point Mutations / Variants
Construct 1: pET28a-pelB-ICCG-DoT
Features: T7 promoter | Lac promoter | Ribosome binding site | PelB signal peptide | ICCG | Linker | Dockerin T | TEV cutting site | 8xHistag
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAAGGAGATATACCATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCAGCAATCCGTATCAGCGTGGTCCGAATCCGACCCGTAGCGCACTGACCGCAGATGGTCCGTTTAGCGTTGCAACCTATACCGTTAGCCGTCTGAGCGTTAGCGGTTTTGGTGGTGGTGTTATTTATTATCCGACCGGTACCAGCCTGACCTTTGGTGGTATTGCAATGAGCCCGGGTTATACCGCAGATGCAAGCAGCCTGGCATGGCTGGGTCGTCGTCTGGCAAGCCACGGTTTTGTTGTTCTGGTTATTAATACCAATAGCCGTTTTGATGGTCCGGATAGCCGTGCAAGCCAGCTGAGCGCAGCACTGAATTATCTGCGTACCAGCAGCCCGAGCGCAGTTCGTGCACGTCTGGATGCAAATCGTCTGGCAGTTGCAGGTCATAGCATGGGTGGTGGTGGTACCCTGCGTATTGCAGAACAGAATCCGAGCCTGAAAGCAGCAGTTCCGCTGACCCCGTGGCATACCGATAAAACCTTTAATACCAGCGTTCCGGTTCTGATTGTTGGTGCAGAAGCAGATACCGTTGCACCGGTTAGCCAGCATGCAATTCCGTTTTATCAGAATCTGCCGAGCACCACCCCGAAAGTTTATGTTGAACTGTGTAATGCAAGCCATATTGCACCGAATAGCAATAATGCAGCAATTAGCGTTTATACCATTAGCTGGATGAAACTGTGGGTTGATAATGATACCCGTTATCGTCAGTTTCTGTGTAATGTTAATGATCCGGCACTGTGTGATTTTCGTACCAATAATCGTCATTGTCAGACTAGTGGAGGAGGTGACGACGGTGGAAGCGGTGGTCCTCAAGGTACCACATATAAAGTACCTGGTACTCCTTCTACTAAATTATACGGCGACGTCAATGATGACGGAAAAGTTAACTCAACTGACGCTGTAGCATTGAAGAGATATGTTTTGAGATCAGGTATAAGCATCAACACTGACAATGCCGATTTGAATGAAGACGGCAGAGTTAATTCAACTGACTTAGGAATTTTGAAGAGATATATTCTCAAAGAAATAGATACATTGCCGTACAAGAACGAGCTCGAGAACCTTTACTTCCAAGGTCACCACCATCATCATCACCATCATTAA
Construct 2: pET28a-TfCa-doG (Wild Type)
Features: T7 promoter | Lac promoter | Ribosome binding site | TfCa | Linker | Dockerin G | TEV cutting site | 8xHistag
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAAGGAGATATACCATGGAAATTGTTATCCGGACAGGGAGTGGTGACGTTCGCGGATCAAAGGAGAATGGAATCGCTGTGTTCAGAGGCATCCCGTACGCAGAGCCACCGGTAGGTGCACATAGATTTACGGCCCCTAGACCACCGCGGCCATGGGACGGTGTGCGTGATGCGACGGAGTTCTCTGCTACCGCCCCTAGACCACCATATCCTGAGGCAATAGGCGCCCTTCTGATCGAGCGCTTCATCCCTGGCGACGATTATCTGACACTGAACGTATGGACTCCAGACCCTAATGCGGTGGGTCTGCCTGTCATGGTGTGGATACATGGCGGAGCCTTCACTAACGGATCCGGTTCGGAGCCTGTTTACGACGGTGCGGCATTTGCGAGAGACGGAGTGGTCTTCGTGTCGTTTAATTACCGTTTGGGTATCATTGGCTTCGCGGATTTGCCTGACGCACCTTCGAACAGAGGCCTGCTGGATCAAATTGCTGCACTGGAGTGGGTCCGGGACAACATCGCTCGATTCGGCGGTGATCCGGGTAACGTGACCGTATTCGGGGAGAGTGCCGGCGCTATGAGCGTCTGCACCTTAATGGCCACCCCAAGAGCTCGTGGCTTGTTTAGACGAGCCATTCTTCAGAGTGGTGCAGGGAATATGGCCGTAGCCGCTGAGGACGCGACAACGATAGCAGCAGTTATTGCTCACCGCTTAGGTGTCGAACCAACGGCTGCCGCCCTGGCCCATGTGCCCGTCGCCCAATTGCTGGATGTCCAACAGCAGGTAGCCCAGGAGATACAAGGAGCGCCTGATCCAGCCGTTTGGGGAGAACGGATCGCCGGCGGTTCTGTCTTACTGCCATTTGCTCCTGTCATCGACGGTGAGTTGCTTAGCCAACGTCCCGCCGAGGCCATTGCGGGCGGTGCCGGGCACGATGTTGACCTGCTGTTTGGAACCACGACAGACGAGTATAGATTGTTCTTGGCGCCAACAGGACTTCTGCCATTTATTACCAGTGACTATGTTACTGCACATCTTGCCAAATCCGGGCTGGACGCAGATGCAGCAAAAGCATACACGGCGGAAGGCCGTGGCGAAGAGCCCGGCGATATCCTGGCATCGATTATAACCGACCAAGTATTCAGAATACCTGCCTTGAGAATTGCTGAGTCCCGGGTTGACGCTCCTGCTCGGACGTTTGGGTATGAGTTTGCGTGGCGTACTCCACAACTCGATGGTATTCTTGGAGCGTGCCACGCCGTTGAGCTGCCCTTCGTATTTCGCACCTTAGACCGTGCCGCCAGTCTTGTTGGAACCAACCCACCGGAAGAGTTAGCCGAGACCGTACACAATGCCTGGGT

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