Engineering Success
Our iterative design process and laboratory cycles.
01
Initial Circuit Design
Design
We designed a genetic toggle switch using the LacI and TetR repressors...
Build
Gibson Assembly was used to clone the circuit into pSB1C3.
Test
Fluorescence was measured over 12 hours via plate reader.
Learn
The promoter strength was too high, causing metabolic burden.
02
Optimizing Promoter Strength
Based on Cycle 1, we introduced a library of RBS variants...