Results

Our microbial cell factories produced ICCG-DoT, TfCa-DoG, and ScafGVT.

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• Composite Part Was Mapped. The Composite Part has its own page.
• The Measurement was Excellent. The Measurement has its own page.

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• Clearly and objectively describe the results of your experiments and research.
• Use tables, graphs, and images to visualize your data.
• Discuss the implications of your findings in the context of your project goals.
• Highlight any trends, patterns, or significant observations.
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• Explain what went wrong and what you learned from these experiences.
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Jett's Version: Results, with error bars.

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The section below is a separate, independently-written draft, produced by a team member outside the main documentation process. Kept here for reference and comparison; not yet fact-checked by team leadership.

Three measured numbers and three modelled numbers, kept explicitly separate throughout — on the argument that conflating measured and modelled results is the most common form of scientific dishonesty in undergraduate-style writing.

Headline numbers

Metric	Value	Source
PETase expression (soluble)	2.1 ± 0.6 mg/L	Measured (n=3)
Lpp-OmpA-PETase surface activity	+34% vs. soluble	Measured (n=3)
Kill-switch A escape	~10⁻⁴	Measured (day-1 plates)
Kill-switch B escape	~10⁻³	Measured (day-1 plates · needs work)
PET conversion (full bioreactor)	87%	Modelled (kinetic sim)
CO₂ fixed per reactor day	240 g/d	Modelled (stretch only)

PETase expression & surface display

SDS-PAGE densitometry across three independent transformations. The Lpp-OmpA fusion gives slightly lower total titre than soluble expression (1.8 vs. 2.1 mg/L), but the displayed fraction shows 34% higher activity on PET film — suggesting the surface-displayed form has better substrate access. Ag43 was toxic at activity-relevant levels; INPNC is marginally viable (membrane stress at high induction). Lpp-OmpA continues as the primary anchor.

PET-film hydrolysis

Standard 100 mg PET film discs, 25°C, 14 days, quantified by mass loss + HPLC monomer detection. Detectable mass loss in enzyme-bearing samples; sub-detection-limit in negative controls.

Kill-switch escape

Switch A (thermosensitive lambda): ~10⁻⁴ escape rate at day 1. Switch B (light-activated colicin): ~10⁻³, worse than predicted. Joint escape rate, assuming orthogonality: ~10⁻⁵ — the independence assumption is still being tested by co-induction experiments.

What's missing

Not yet measured: long-term (90-day) viability of the production strain under dormancy; PET-film mass loss at bioreactor-cartridge scale (only assayed at 24-well-plate scale so far); independence of the two kill switches under joint stress.

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