Present the results of your project, along with a detailed analysis and discussion of their significance. Also outline future plans and reflections on the impact of your project.
The section below is a separate, independently-written draft, produced by a team member outside the main documentation process. Kept here for reference and comparison; not yet fact-checked by team leadership.
Three measured numbers and three modelled numbers, kept explicitly separate throughout — on the argument that conflating measured and modelled results is the most common form of scientific dishonesty in undergraduate-style writing.
| Metric | Value | Source |
|---|---|---|
| PETase expression (soluble) | 2.1 ± 0.6 mg/L | Measured (n=3) |
| Lpp-OmpA-PETase surface activity | +34% vs. soluble | Measured (n=3) |
| Kill-switch A escape | ~10⁻⁴ | Measured (day-1 plates) |
| Kill-switch B escape | ~10⁻³ | Measured (day-1 plates · needs work) |
| PET conversion (full bioreactor) | 87% | Modelled (kinetic sim) |
| CO₂ fixed per reactor day | 240 g/d | Modelled (stretch only) |
SDS-PAGE densitometry across three independent transformations. The Lpp-OmpA fusion gives slightly lower total titre than soluble expression (1.8 vs. 2.1 mg/L), but the displayed fraction shows 34% higher activity on PET film — suggesting the surface-displayed form has better substrate access. Ag43 was toxic at activity-relevant levels; INPNC is marginally viable (membrane stress at high induction). Lpp-OmpA continues as the primary anchor.
Standard 100 mg PET film discs, 25°C, 14 days, quantified by mass loss + HPLC monomer detection. Detectable mass loss in enzyme-bearing samples; sub-detection-limit in negative controls.
Switch A (thermosensitive lambda): ~10⁻⁴ escape rate at day 1. Switch B (light-activated colicin): ~10⁻³, worse than predicted. Joint escape rate, assuming orthogonality: ~10⁻⁵ — the independence assumption is still being tested by co-induction experiments.
Not yet measured: long-term (90-day) viability of the production strain under dormancy; PET-film mass loss at bioreactor-cartridge scale (only assayed at 24-well-plate scale so far); independence of the two kill switches under joint stress.
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