Composite Part

Our project sought to advance the synthetic biology of composite parts.

Our Composite Part

We hope our composite part will be useful to future teams.

Sequence Feature Legend

T7 / Lac Promoters
Ribosome Binding Site (RBS)
Signal Peptides (pelB)
Enzymes / Domains (ICCG, TfCa)
Linkers / Spacers
Interaction Modules (Dockerin / Cohesin)
Cleavage Sites (TEV) & Purification (Histag)
Point Mutations / Variants
Construct 1: pET28a-pelB-ICCG-DoT
Features: T7 promoter | Lac promoter | Ribosome binding site | PelB signal peptide | ICCG | Linker | Dockerin T | TEV cutting site | 8xHistag
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAAGGAGATATACCATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCAGCAATCCGTATCAGCGTGGTCCGAATCCGACCCGTAGCGCACTGACCGCAGATGGTCCGTTTAGCGTTGCAACCTATACCGTTAGCCGTCTGAGCGTTAGCGGTTTTGGTGGTGGTGTTATTTATTATCCGACCGGTACCAGCCTGACCTTTGGTGGTATTGCAATGAGCCCGGGTTATACCGCAGATGCAAGCAGCCTGGCATGGCTGGGTCGTCGTCTGGCAAGCCACGGTTTTGTTGTTCTGGTTATTAATACCAATAGCCGTTTTGATGGTCCGGATAGCCGTGCAAGCCAGCTGAGCGCAGCACTGAATTATCTGCGTACCAGCAGCCCGAGCGCAGTTCGTGCACGTCTGGATGCAAATCGTCTGGCAGTTGCAGGTCATAGCATGGGTGGTGGTGGTACCCTGCGTATTGCAGAACAGAATCCGAGCCTGAAAGCAGCAGTTCCGCTGACCCCGTGGCATACCGATAAAACCTTTAATACCAGCGTTCCGGTTCTGATTGTTGGTGCAGAAGCAGATACCGTTGCACCGGTTAGCCAGCATGCAATTCCGTTTTATCAGAATCTGCCGAGCACCACCCCGAAAGTTTATGTTGAACTGTGTAATGCAAGCCATATTGCACCGAATAGCAATAATGCAGCAATTAGCGTTTATACCATTAGCTGGATGAAACTGTGGGTTGATAATGATACCCGTTATCGTCAGTTTCTGTGTAATGTTAATGATCCGGCACTGTGTGATTTTCGTACCAATAATCGTCATTGTCAGACTAGTGGAGGAGGTGACGACGGTGGAAGCGGTGGTCCTCAAGGTACCACATATAAAGTACCTGGTACTCCTTCTACTAAATTATACGGCGACGTCAATGATGACGGAAAAGTTAACTCAACTGACGCTGTAGCATTGAAGAGATATGTTTTGAGATCAGGTATAAGCATCAACACTGACAATGCCGATTTGAATGAAGACGGCAGAGTTAATTCAACTGACTTAGGAATTTTGAAGAGATATATTCTCAAAGAAATAGATACATTGCCGTACAAGAACGAGCTCGAGAACCTTTACTTCCAAGGTCACCACCATCATCATCACCATCATTAA
Construct 2: pET28a-TfCa-doG (Wild Type)
Features: T7 promoter | Lac promoter | Ribosome binding site | TfCa | Linker | Dockerin G | TEV cutting site | 8xHistag
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAAGGAGATATACCATGGAAATTGTTATCCGGACAGGGAGTGGTGACGTTCGCGGATCAAAGGAGAATGGAATCGCTGTGTTCAGAGGCATCCCGTACGCAGAGCCACCGGTAGGTGCACATAGATTTACGGCCCCTAGACCACCGCGGCCATGGGACGGTGTGCGTGATGCGACGGAGTTCTCTGCTACCGCCCCTAGACCACCATATCCTGAGGCAATAGGCGCCCTTCTGATCGAGCGCTTCATCCCTGGCGACGATTATCTGACACTGAACGTATGGACTCCAGACCCTAATGCGGTGGGTCTGCCTGTCATGGTGTGGATACATGGCGGAGCCTTCACTAACGGATCCGGTTCGGAGCCTGTTTACGACGGTGCGGCATTTGCGAGAGACGGAGTGGTCTTCGTGTCGTTTAATTACCGTTTGGGTATCATTGGCTTCGCGGATTTGCCTGACGCACCTTCGAACAGAGGCCTGCTGGATCAAATTGCTGCACTGGAGTGGGTCCGGGACAACATCGCTCGATTCGGCGGTGATCCGGGTAACGTGACCGTATTCGGGGAGAGTGCCGGCGCTATGAGCGTCTGCACCTTAATGGCCACCCCAAGAGCTCGTGGCTTGTTTAGACGAGCCATTCTTCAGAGTGGTGCAGGGAATATGGCCGTAGCCGCTGAGGACGCGACAACGATAGCAGCAGTTATTGCTCACCGCTTAGGTGTCGAACCAACGGCTGCCGCCCTGGCCCATGTGCCCGTCGCCCAATTGCTGGATGTCCAACAGCAGGTAGCCCAGGAGATACAAGGAGCGCCTGATCCAGCCGTTTGGGGAGAACGGATCGCCGGCGGTTCTGTCTTACTGCCATTTGCTCCTGTCATCGACGGTGAGTTGCTTAGCCAACGTCCCGCCGAGGCCATTGCGGGCGGTGCCGGGCACGATGTTGACCTGCTGTTTGGAACCACGACAGACGAGTATAGATTGTTCTTGGCGCCAACAGGACTTCTGCCATTTATTACCAGTGACTATGTTACTGCACATCTTGCCAAATCCGGGCTGGACGCAGATGCAGCAAAAGCATACACGGCGGAAGGCCGTGGCGAAGAGCCCGGCGATATCCTGGCATCGATTATAACCGACCAAGTATTCAGAATACCTGCCTTGAGAATTGCTGAGTCCCGGGTTGACGCTCCTGCTCGGACGTTTGGGTATGAGTTTGCGTGGCGTACTCCACAACTCGATGGTATTCTTGGAGCGTGCCACGCCGTTGAGCTGCCCTTCGTATTTCGCACCTTAGACCGTGCCGCCAGTCTTGTTGGAACCAACCCACCGGAAGAGTTAGCCGAGACCGTACACAATGCCTGGGT

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